| 李云霞,韩根亮,王艳凤,马莉萍.化学通报,2014,77(10):1013-1016. |
| 基于石墨烯/纳米金/壳聚糖复合膜固定化酶的有机磷生物传感器 |
| A organophosphates Biosensor Based on RGO/AuNPs /CS composite membraneImmobilized Enzyme |
| 投稿时间：2014-01-16 修订日期：2014-03-05 |
| DOI： |
| 中文关键词: 乙酰胆碱酯酶,石墨烯,电化学生物传感器,有机磷农药,乐果 |
| 英文关键词:acetylcholinesterase, graphene, electro-biosensor, organophosphates, Rogor. |
| 基金项目:(2013QN-04、2012QN-06),兰州市科技计划项目(2012-2-61),甘肃省青年科技基金计划(1107RJYA034)资助 |
|
| 摘要点击次数: 2935 |
| 全文下载次数: 0 |
| 中文摘要: |
| 将乙酰胆碱酯酶(AChE)固定到石墨烯(RGO)/ 纳米金(AuNPs)/壳聚糖(CS)复合膜修饰的玻碳电极表面,制备了高灵敏度的电化学生物传感器用于有机磷农药的检测。由于石墨烯具有较大的比表面积和良好的导电性,复合材料不仅为保持AChE的生物活性提供了良好的生物相容环境,而且RGO/AuNPs的协同作用也提高了传感器的灵敏度。CS/SiO2复合溶胶-凝胶网格状的结构为酶的固定化提供了良好的载体。农药抑制率与乐果浓度的负对数在0.1～10.0 ng/mL范围内呈线性关系,检出限为0.02 ng/mL(S/N=3)。 |
| 英文摘要: |
| A highly sensitive organophosphates (OPs) electro-biosensor was developed by immobilizing acetylcholinesterase (AChE) to graphene(RGO)/gold nanoparticles (AuNPs)/ chitosan (CS)composite on glass-carbon electrode. Because of the higher specific surface area and high conductivity of RGO, composite material not only provided a biocompatible environment to keep the bioactivity of AChE, but also RGO/AuNPs exhibited a strong synergetic effect on improving the sensing properties of OPs. CS/SiO2 sol-gel offered a good supporter to enzyme because of its meshy structure. The linear relationship between the inhibition of rogor and its negative logarithm of concentration was found in the range of 0.1~10.0 ng/mL, with a detection limit of 0.02 ng/mL ( S/N= 3). |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|