| 张露露,张松,孙瑞成,刘新利.化学通报,2015,78(1):89-92. |
| 纳米羟基磷灰石负载Epothilone B的细胞活性研究 |
| Anticancer activity of surface modified epothilone B attached nano-hydroxyapatite |
| 投稿时间：2014-05-05 修订日期：2014-06-20 |
| DOI： |
| 中文关键词: 羟基磷灰石纳米颗粒 Epothilone B 药物载体 肿瘤细胞，抗肿瘤活性 |
| 英文关键词:Hydroxyaptite nanoparticles, Epothione B, Drug carriers, Cancer cells, Anti-tumor activity |
| 基金项目:山东省科技发展计划 (2012GSF12107) |
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| 中文摘要: |
| 羟基磷灰石纳米材料因其生物相容性好，比表面积大，可以吸附大量的药物分子，因此在纳米医药领域极具有应用前景。本文通过共沉淀法制备出纳米羟基磷灰石，然后利用x-射线衍射，傅里叶红外，扫描电镜，透射电子显微镜进行表征，结果表明，所制备的纳米羟基磷灰石呈针形，长约70 ~ 90 nm。实验表明，采用二氯甲烷作为溶剂，EpoB搭载效率最高，最大搭载量达0.53 g/(g HA)。应用MTT法对纳米羟基磷灰石及复合物的抗肿瘤活性进行分析，结果显示HA-EpoB复合物对肺癌细胞NCI-H292和LLC的半抑制率浓度分别为15 ng/mL和35 ng/mL，明显低于游离EpoB的半抑制率35 ng/mL和50 ng/mL。这可能是纳米羟基磷灰石能够更容易的进入细胞内，然后将药物释放，进而抑制肿瘤细胞生长。 |
| 英文摘要: |
| Hydroxyapatite materials (HA) have shown applications in biomedicine, because of the high biocompatibility and large surface area. Hydroxyapatite nanoparticles were synthesized by co-precipitation in this paper, and the nanoparticles are characterised with x-ray diffraction(XRD), Fourier transform infrared(FTIR), scanning electron microscopy(SEM) and transmission electron microscopy(TEM), the results show that hydroxyapatite nanoparticles in shape of needles with a length of 70 ~ 90 nm. The maximum carrying capacity, 0.53 g/(g HA), was obtained in methylene chloride for EpoB. The cytotoxicity of HA, EpoB and HA-EpoB on NCI-H292 and LLC cells were determined by the MTT assay. The IC50 value for HA-EpoB is less than that for EpoB. Lung cancer cells NCI-H292 and LLC, the IC50 value is 15 ng/mL and 35 ng/mL, respectively. This may be contributed to nano-hydroxyapatite can enter the cells easily, release the drugs in intracellular, and then inhibit inhibit the growing of cancer cells. |
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