| 李振华,陈绪煌,尹应乐,刘最芳.化学通报,2015,78(3):196-201. |
| 酶电极电子转移的途径及研究进展 |
| Approaches and Progresses in Facilitating Electron Transfer in Enzymatic Electrodes |
| 投稿时间：2014-07-16 修订日期：2014-09-09 |
| DOI： |
| 中文关键词: 酶电极 生物传感器 电介体 氧化还原聚合物 电子转移 |
| 英文关键词:Enzymatic electrode Biosensor Mediator Redox polymer Electron transfer |
| 基金项目:国家自然科学基金项目(51303049)资助 |
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| 摘要点击次数: 2765 |
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| 中文摘要: |
| 酶的活性中心与电极表面的电子转移直接影响酶电极的性能和特征。自从1962年第一个酶电极报道以来,科学家们不断探索新的方式实现酶与电极间电子转移并取得了较大的进展,使生物传感器由第一代依靠氧与葡萄糖氧化酶中的活性中心反应,测量氧的消耗为原理,发展到第三代实现酶的活性中心与电极表面之间的直接电子转移,即所谓的“无试剂电化学生物传感器”。然而,探究酶电极内在电子转移机理以及设计能够满足不同应用要求并适合大规模量产、价格合适的酶电极仍然是研究的热点。本文综述了主要的电子转移方式以及相应的优缺点,以及作者的研究小组开发的使用氧化还原聚合物实现电子转移的方法,并对其应用前景进行展望。 |
| 英文摘要: |
| Performances and characteristics of an enzymatic electrode are directly related to electron transfer between the enzyme active centre and the electrode surface. Since the first enzymatic electrode reported in 1962, tremendous progresses have been made in facilitating the electron transfer through a variety of approaches, leading to the development of three generations of enzymatic electrochemical biosensors. However, challenges remain in understanding the underlying mechanisms and in designing enzymatic electrodes which fulfill various application requirements and suitable for mass production at reasonable costs. This review provides an overview of the main electron transfer approaches, the related methodologies and their merits and limitations. Special emphasis is given to the recent research work of author’ team and those reported both abroad and at home in the area of direct electron transfer, in particular the use of redox polymers. Major considerations for the design and fabrication of redox polymer modified enzymatic electrodes and further researches are discussed. |
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