| 庞向东,冉健康,江虹.化学通报,2015,78(2):190-192. |
| 头孢西丁与甲基绿的显色反应及应用 |
| Color Reactions of Cefoxitin with Methyl Green and Their Analytical Applications |
| 投稿时间：2014-07-18 修订日期：2014-08-27 |
| DOI： |
| 中文关键词: 甲基绿, 头孢西丁, 显色反应, 分光光度法 |
| 英文关键词:Methyl green Cefoxitin Color reaction Spectrophotometry |
| 基金项目:重庆市教委科技基金资助项目(KJ1401226)资助 |
|
| 摘要点击次数: 2744 |
| 全文下载次数: 0 |
| 中文摘要: |
| 在pH 6.35 的 Tris-HCl 缓冲溶液中,头孢西丁与甲基绿反应生成具有两个明显正吸收峰的离子缔合物,最大和次大吸收波长分别位于664 nm 和560 nm,表观摩尔吸光系数分别为 2.98×104 和1.76×104 L/(mol.cm),头孢西丁的质量浓度在0.85～11.12 mg/L 以内遵守比尔定律。方法用于人体血液及药物中头孢西丁含量的测定,加标回收率在 99.1%～101.2% 之间,相对标准偏差在 1.6%～2.0% 之间,测定值与标示量一致。 |
| 英文摘要: |
| In Tris-HCl buffer solution of pH 6.35, cefoxitin (CEXI) reacts with methyl green (MEG) to form an ion-association complex with two obvious positive absorption peak. The maximum positive absorption wavelength and sub-major positive absorption wavelength were 664 nm and 560 nm respectively, and apparent molar absorptivity (ε) were 2.98×104 and 1.76×104 L/(mol.cm), and Beer’s law was obeyed in the range within 0.85~11.12 mg/L of cefoxitin. The method was applied to determine the content of cefoxitin in commercially available cefoxitin sodium medicament and in human blood, and results obtained were in conformity with labeled values. Values of recovery by standard addition method was in the range of 99.1%～101.2% and RSD＇s (n=6) in the range of 1.6%～2.0%. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|