| 周殿芳,陈建武,彭婕,甘金华,何力.化学通报,2015,78(5):467-470. |
| UPLC法检测渔用饲料中八种磺胺类药物残留的研究 |
| The study of determination of SAs in fish formula feed by HPLC |
| 投稿时间：2014-10-08 修订日期：2014-10-29 |
| DOI： |
| 中文关键词: 渔用饲料 磺胺类药物 残留 超高效液相色谱 |
| 英文关键词:formula feed SAs residue UPLC |
| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| 中文摘要: |
| 本文通过对多组样品的试验测定,建立了一种渔用饲料中8种常用磺胺(磺胺嘧啶、磺胺甲基嘧啶、磺胺二甲基嘧啶、磺胺噻唑、磺胺甲基异恶唑、磺胺多辛、磺胺异恶唑和磺胺喹恶啉)的超高效液相色谱检测法。样品经2%乙酸浸润,4℃预冷的乙腈提取,碱性氧化铝柱净化,用Symmetry-C18色谱柱(5μm,4.6×150mm),2%乙酸-甲醇梯度洗脱,检测波长270nm。结果表明：8种磺胺的定量底限为2mg/kg,在0.5-20μg/mL内,线性关系良好,R2＞0.99。添加水平10mg/kg时,八种磺胺类药物的平均回收率范围为70-100%,RSD＜15。该方法回收率高,简单,准确可靠,可用于渔用饲料中磺胺类药物残留的监测。 |
| 英文摘要: |
| Through experimental determination of multiple sets of samples, A liquid Chromatographic method has been developed for determination of 8(SD、ST、SM1、SM2、SMZ、ADM’、SFZ、SQX)sulfonamides in formula feed.The method involves using 2% acetic acid infiltration and acetonitrile to extract sulfonamides from feed matrix.a Alumina-B SPE cartridge to isolate the SAs,With Symmetry-C18 column(5μm, 4.6×150mm), 2% acetic acid-methanol gradientdetection by wavelength 270nm. The minimum limit of quantitation is 2mg/kg for 8 sulfonamides,between 0.5μg/mL to 20μg/mL,The linear relationship was good,R2＞0.99.For spiked feed sample,recovery is 70-95%,the RSDs ＜10.The high recovery method is simple, accurate and reliable, and can be used to monitor SAs residue in formula feed. |
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