| 刘 蓉,龙立平,刘石泉,赵运林,颜 徐,李 杰.化学通报,2015,78(10):918-922. |
| 电聚合分子印迹传感器选择性测黑茶中槲皮素的含量 |
| Electrochemical Polymerization Molecularly Imprinted Sensor for Selective Determination with Quercetin of Black Tea |
| 投稿时间：2014-11-20 修订日期：2015-01-26 |
| DOI： |
| 中文关键词: 槲皮素 分子印迹 差分脉冲伏安法 黑茶 |
| 英文关键词:Quercetin Molecular imprinting Differential pulse voltammetry Black tea |
| 基金项目:湖南省科技计划项目( 2012GK3070 )，湖南省应用基础研究基地开放基金项目(湘科计字2014HCKF03)，湖南省科学技术厅国际合作项目(2012WK2013)，湖南省高校科技创新团队持计划资助项目(2014-11)，益阳市指导性科技计划项目(2014JZ26 )，湖南省教育厅高校科研一般项目(15C0244)。 |
|
| 摘要点击次数: 2543 |
| 全文下载次数: 0 |
| 中文摘要: |
| 以槲皮素作为模板分子,邻氨基酚作为功能单体,在金电极表面通过电聚合法,制备了具有特异性识别槲皮素分子印迹传感器膜(MIP)。采用循环伏安法(CV)、差分脉冲伏安法(DPV)研究了印迹膜的性能、结构和分子印迹效应。对实验参数,如：功能单体与模板分子的配比、洗脱时间、印迹时间等进行了优,并与其结构相似的化合物芦丁的选择性响应进行了比较,发现该传感器对槲皮素分子具有良好的选择性。槲皮素浓度在6.0×10-6~1.0×10-4 mol/L范围内与峰电流呈线性关系,线性方程为：I(μA)=27.7886+ 9.4752logc (mol/L)S(R=0.99387),检出限为2.0×10-6 mol/L。该传感器测得了黑茶中槲皮素的含量,结果较为满意。 |
| 英文摘要: |
| Electrochemical polymerization imprinted membrane (MIP) sensor for quercetin was fabricated with specific recognitionScavities. ItSwas prepared by using quercetinSas the template molecule,So-aminophenolSas functional monomerSon the surface of the gold electrode. TheSimprinted membrane performance,Sstructure and molecularSimprinting effection was studied by using the cyclic voltammetryS(CV) and differential pulseSvoltammetryS(DPV). The sensor exhibited good selectivity to quercetin with the interferences about rutin which has the similar structure as quercetin. The peak current has a linear relationship with quercetinSconcentration within the the range of 6.0×10-6~1.0×10-4mol/L, and the linear equation was I(μA)=71.3286 9.47519logc (mol/L)S (R=0.99387) with detection limit of 2.0×10-6 mol/L. It was successfully applied for the determination of rutin in Black tea. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |