| 熊喜悦,盛小奇,王华,王鷁超.化学通报,2015,78(7):602-607. |
| 代谢组学GC-MS分析方法中样品衍生化技术的新进展 |
| Development of chemical derivatization in metabolites analysis by GC-MS |
| 投稿时间：2014-12-01 修订日期：2015-01-20 |
| DOI： |
| 中文关键词: 代谢组学 化学衍生 肟化-硅烷化 烷基化 衍生效率 |
| 英文关键词:metabolomics chemical derivatization tandem oximation-silylation alkylation derivatization efficiency |
| 基金项目:湖南省科技厅专项2013XC83-11和2014SK4044 |
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| 中文摘要: |
| 代谢组学是通过考察生物体系受刺激或扰动前后(如某个特定的基因变异或环境变化后)代谢产物的动态变化, 研究生物体系代谢网络的一种技术,其研究对象主要是内源性小分子物质。这些物质大部分极性强,难挥发,在GC-MS分析前,需要进行适当的化学衍生处理。近几年来化学衍生技术发展迅速,本文主要从衍生试剂种类、衍生条件和衍生效果(包括衍生效率、重复性和产物稳定性等)出发,综述了已报道的衍生方法的特点,并展望了衍生技术的发展前景。 |
| 英文摘要: |
| Metabolomics involves the holistic analysis of the changes in the complete set of metabolites (small organic compounds, MW < 1000) in the cell, body fluids, or tissue. However, many metabolites contain polar functional groups that are thermally labile at the temperature required for their separation by GC-MS or are not volatile at all. Therefore, suitable derivatization of these compounds prior to GC-MS analysis is necessary. Herein, based on derivatization reagents, reaction conditions, derivatization efficiencies and stability of the derivatives, derivatization methods are comprehensively summarized and future development prospects were presented. |
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