| 聂立波,麻鹏,肖湘云,王秀利.化学通报,2015,78(9):785. |
| 基于金纳米棒的DNA超灵敏检测研究 |
| Ultra-sensitive DNA Detection based on Gold Nanorods |
| 投稿时间：2015-01-23 修订日期：2015-03-04 |
| DOI： |
| 中文关键词: 金纳米棒 表面等离子体共振 寡核苷酸 杂交 |
| 英文关键词:Gold nanorods, Surface plasmon resonance, Oligonucleotide, Hybridization |
| 基金项目: |
|
| 摘要点击次数: 2379 |
| 全文下载次数: 3136 |
| 中文摘要: |
| 采用氯金酸和硼氢化钠为原料合成金纳米粒子,在含有CTAB的生长溶液中加入一定量的种子溶液成长形成金纳米棒。在金纳米棒表面分别组装两种不同的巯基寡核苷酸探针,并通过靶探针进行“三明治”杂交。采用透射电子显微镜(TEM)、紫外-可见-近红外光谱对杂交体系进行了表征。研究结果表明,所制备的金纳米棒分布均匀、分散性好,对寡核苷酸的检测限可达1 pM,能明显区分正错配。该方法简单易行,可应用于DNA、蛋白质等生物分子的识别。 |
| 英文摘要: |
| The gold nanoparticle seeds were prepared via the reduction of HAuCl4 by NaBH4, then the gold nanorods were synthesized based on the growth of gold nanoparticle seeds. Thiol-oligonucleotide probes were immobilized on the surface of gold nanorods, and a “sandwich” hybridization was performed by the hybridization of target oligonucleotides and thiol-oligonucleotide probes. The hybridization system was investigated by TEM and UV-vis-near-IR absorption spectra. The results showed that gold nanorods were of good dispersion. The detection limit of target DNAs was 1 pM, and the complementary and mismatched DNAs can be distinguished clearly. This simple method could be used in the recongnization of DNAs and proteins. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|