| 谢兵,刘孟臣,周杰,朱树华.化学通报,2015,78(11):1033-1038. |
| 一氧化氮对ACC 氧化酶活性的抑制动力学研究 |
| Study on the inhibition kinetics of NO on ACO activity |
| 投稿时间：2015-03-16 修订日期：2015-05-27 |
| DOI： |
| 中文关键词: 一氧化氮 ACC氧化酶 抑制作用 动力学 |
| 英文关键词:nitric oxide, ACC oxidase, inhibition, kinetic |
| 基金项目:国家自然科学基金项目 |
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| 中文摘要: |
| NO可以通过其辅因子氧化灭活作用抑制ACC氧化酶(ACO) 活性, 从而降低乙烯释放速率。本文主要研究了NO对ACO抑制作用的化学动力学,揭示了NO对ACO活性的抑制机理。NO溶液与ACC氧化酶的最佳反应时间是30 min,底物ACC的最佳浓度为2 mmol L-1,作为酶促反应的辅因子,抗坏血酸钠和Fe2＋的最适浓度分别为60 mmol L-1和60 μmol L-1,碳酸氢钠的最适浓度为60 mmol L-1。Lineweaver-Burk图显示NO是一种非竞争性抑制剂。NO与ACC氧化酶反应的米氏常数Km为2.54 mmol L-1,抑制常数Ki为3.22 μmol L-1。 |
| 英文摘要: |
| NO can restrain the activity of ACO via the inactivate oxidation of NO’s cofactor, resulting reduction of ethylene producing rate. This paper mainly studied the inhibition kinetics of NO on ACO activity, revealed the inhibition mechanism of NO on ACO activity. The proper time of reaction between NO and ACO was 30 min, and proper concentration of ACC is 2 mmol L-1. As cofactors of the enzymic reaction, the proper concentrations of Vc, NaHCO3 and Fe2＋were 60 mmol L-1, 60 mmol L-1 and 60 μmol L-1 respectively. The inhibition kinetics of NO analyzed by Lineweaver-Burk plots demonstrated that the inhibition effect of NO for the oxidation of ACO expressed as what a noncompetitive inhibitor does. The apparent Michaelis constant Km and the inhibition constant of noncompetitive reversible inhibitor Ki were determined to be 2.54 mmol L-1 and 3.22 μmol L-1 respectively. |
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