| 常昂,刘祥军,吴尚荣,柳影,王林林,张楠,李松青,上官棣华.化学通报,2015,78(12):1101. |
| 2,9-双甲基苯并噻唑乙烯基邻菲罗琳与DNA 的相互作用及应用研究 |
| Interaction of a Phenanthroline Derivative with DNA and its Application |
| 投稿时间：2015-06-23 修订日期：2015-07-28 |
| DOI： |
| 中文关键词: 邻菲罗琳衍生物 DNA 相互作用 荧光探针 |
| 英文关键词:1,10-phenanthroline DNA interaction Fluorescent probe |
| 基金项目:国家“九七三”计划项目( 批准号: 2011CB935800,2013CB933700) 和国家自然科学基金(批准号：21275149,21375135,21321003) 资助。 |
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| 中文摘要: |
| 设计合成了基于2，9-双苯并噻唑乙烯基取代的邻菲罗琳荧光分子—PMBT。通过吸收光谱、荧光光谱、圆二色光谱探讨了PMBT与不同DNA的相互作用。发现PMBT与DNA存在两种不同的相互作用模式。 由于PMBT分子中带有两个正电荷，当PMBT与DNA的浓度比值较高（大于4）时，PMBT以DNA为模板按一定方向在DNA上聚集；当PMBT与DNA的浓度比小于2时，PMBT通过嵌插或末端堆积的方式分别与单/双链DNA和G-四链体DNA结合。PMBT与DNA结合导致其荧光淬灭，利用该特性将PMBT与DNA结合构建荧光增强型检测平台，可用于DNA酶活性以及DNA降解的动力学研究。 |
| 英文摘要: |
| A phenanthroline derivative, 2,9-bis(2-(3-methylbenzo[d]thiazol-2-yl)vinyl)-1,10- phenanthroline (PMBT), was designed and synthesized. The interaction of PMBT with different DNA forms was investigated by UV-Vis absorption spectrum, fluorescence spectrum and circular dichroism spectrum. PMBT possesses a large conjugated planar structure, and binds to DNA with different modes dependent on the molecular ratio of PMBT and DNA. At high molecular ratio (>4), PMBT binds to DNA through DNA-templated aggregation because of the two positive charges on each of PMBT. At low molecular ratio (<2), PMBT binds to DNA through intercalation between the DNA bases or end-stacking on G-quadruplexes. PMBT has good solubility in aqueous solution, and exhibits strong fluorescence. The fluorescence is quenched when bound to DNA. Thus PMBT was used as a fluorescent probe for investigation of DNA degradation in serum and the activity of nuclease. |
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