| 苏心怡,刘敏,袁孟娟,韩军.化学通报,2016,79(4):344-348. |
| 不同pH下人血清白蛋白热变性及结构可逆性研究 |
| Study on Thermal Stability of Human Serum Albumin at Different pH Values and Its Structural Reversibility |
| 投稿时间：2015-09-04 修订日期：2015-10-14 |
| DOI： |
| 中文关键词: 差示扫描量热法 动态光散射 圆二色谱 粒径 人血清白蛋白 |
| 英文关键词:DSC,Dynamic light scattering(DLS),Circular dichroism(CD),Particalsize,Human serum albumin(HSA) |
| 基金项目:山东省重点研发计划（2015GSF118160） |
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| 中文摘要: |
| 本文通过用差示扫描量热法(DSC)、动态光散射(DLS)和圆二色谱(CD)检测不同pH下人血清白蛋白(HSA)的结构，并观察其随pH变化的可逆性，以探讨不同pH对HSA结构的影响及HSA结构在pH变化下的可逆性。结果表明，pH7.0条件下HSA的热稳定性最好，热变性温度(Tm)为68.17±0.06℃，不同pH条件下HSA三级结构均发生了一定程度的改变，酸性和碱性条件下的HSA调回pH7.0后变性温度接近于pH7.0条件下的Tm值。不同pH条件下的粒径相对于pH7.0条件下增大，调回pH7.0后减小。CD实验表明了pH对HSA二级结构的影响。HSA结构在不同pH下调回中性条件时会重新折叠，但不能恢复中性条件下的结构状态。 |
| 英文摘要: |
| The tertiary structure of human serum albumin(HSA)and the structure-reversibility at different pH values had been studied by DSC,DLS and CD.The results indicated that HSA proves to be the most stable structure at pH7.0,and the melting temperature(Tm) is 68.17±0.06℃.The tertiary structure of HSA is changed at different pH values and the Tmof HSA is very close to the Tm at pH 7.0 when pH is adjusted back to 7.0 from different pH values.The size of HSA is larger at other different pH values than that at pH 7.0 and it gets smaller when pH is adjusted back to 7.0.The influence of pH on the secondary structure of HSA is detected by CD.AlthoughHSA can be refolded when adjusted back to 7.0 from different pH values,it can’t return back to the structure of HSA at pH7.0. |
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