| 徐晓帆,李润土,李萌萌,李端华,陈春燕,罗忠礼.化学通报,2016,79(6):516-522. |
| 自组装短肽作为“实时监控”纳米材料的初步研究 |
| Preliminary study to monitor real-time by using self-assembling peptide nanomaterials |
| 投稿时间：2015-10-13 修订日期：2015-12-17 |
| DOI： |
| 中文关键词: 短肽GFS-15，自组装，圆二色谱，循环伏安法，纳米纤维 |
| 英文关键词:GFS-15 peptide, self-assembly, CD, cyclic voltammetry, nano-fiber |
| 基金项目:国家自然科学基金(31540019和81101417)，高等学校博士学科点专项科研基金项目(20115503120010)和重庆市科委自然科学基金 (cstc2015jcyjBX0072)，重庆医科大学科研培训基金(201417)，重庆医科大学基础医学院基础医学院苗圃基金(JC201514)，抗生素研究与再评价四川省重点实验室开放课题(ARRLKF14-01)资助 |
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| 中文摘要: |
| 本文主要采用圆二色谱仪、原子力显微镜、刚果红染色等来探究手性自组装短肽GFS-15的二级结构、纳米形态和宏观形貌特征；循环伏安曲线、圆二色谱仪来检测浓度、时间、盐离子对它自组装过程的影响。结果表明，它能形成纳米纤维，能和金电极结合且影响其电化学行为，还可以作为细胞的三维培养基质材料。本研究将为生物实时监控，以及纳米生物医学的前沿研究打下基础。 |
| 英文摘要: |
| The secondary structure, nanostructure and macro-feature to peptide GFS-15 were characterized by circular dichroism spectroscopy (CD), atomic force microscopy (AFM) and Congo red dye. We adopted cyclic voltammetry and CD to study the influence of concentration, self-assembly time and ions to the self-assembly process of the peptide. The result shows that this peptide can form nano-fiber, and the nano-fiber can bind to gold electrode, form to the hydrogel support 3D cell culture matirx. 3D cell culture test, and 3D cell culture real-time tests, confirmed the cell strain not only growth in the nano 3D microenvironment, but also self-assembling to gold electrode sensor (nano-gold particles) surface. Our study could inspire cell real-time monitor and nano-biomedical developing. |
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