| 于婵婵,姚立.化学通报,2016,79(4):292-298. |
| 生物单分子力谱技术的研究进展 |
| Advances in Biological Single-molecule Force Spectroscopy |
| 投稿时间：2015-10-22 修订日期：2015-11-28 |
| DOI： |
| 中文关键词: 单分子力谱技术 生物分子 相互作用 |
| 英文关键词:single-molecule force spectroscopy biological molecules intermolecular interaction |
| 基金项目:国家自然科学基金项目(21573250)和中国科学院项目(YZ201424)资助 |
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| 中文摘要: |
| 近年来，单分子力谱技术获得了快速发展和广泛应用。通过单分子力谱技术研究生物分子结构、力学及动力学，在单分子水平上揭示生物分子间相互作用机制，对于深入了解生物分子的特异识别、生化过程以及生物分子结构与功能的关系具有重要意义。本文主要介绍了3 种最常见的单分子力谱技术：原子力显微镜（AFM），光镊（OT）和磁镊（MT）。另外，还侧重从不同力谱技术的原理、发展及应用三个方面简要介绍了3种大规模并行测量的单分子力谱技术：声力谱（AFS）、离心力显微镜（CFM）以及力诱导剩磁谱技术（FIRMS）。 |
| 英文摘要: |
| In recent years, single-molecule force spectroscopy has been developed rapidly and applied in various fields. The investigation of biological structure, mechanical property, and molecular dynamics by single-molecule force spectroscopycan reveal the mechanism of intermolecular interaction at the single-molecule level. It will greatly help us understand the molecular recognition, biochemical process and the relationship between molecular structure and functions. This paper introduces three kinds of the most common single-molecule force spectroscopy: atomic force microscopy (AFM), optical tweezers (OT), and magnetic tweezers (MT) at first. Then the basic principle, development, and applications of other three kinds of massively parallel force spectroscopy: acoustic force spectroscopy (AFS), centrifugal force microscope (CFM), and force-induced remnant magnetization spectroscopy (FIRMS) are introduced briefly. |
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