| 付建平,韩晓丹,胡居吾,王慧宾,熊伟,李雄辉.化学通报,2016,79(11):1052-1057. |
| 木瓜蛋白酶催化的不对称杂环酮 Aldol反应研究 |
| Papain-catalyzed Asymmetric Aldol Reactions of Heterocyclic Ketones with Aldehydes |
| 投稿时间：2016-04-14 修订日期：2016-06-20 |
| DOI： |
| 中文关键词: 生物催化多功能性 木瓜蛋白酶 Aldol反应 杂环酮 手性合成 |
| 英文关键词:biocatalytic promiscuity papain Aldol reaction heterocyclic ketones asymmetric synthesis |
| 基金项目:国家自然科学基金项目（No.31260400） |
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| 中文摘要: |
| 采用木瓜蛋白酶多功能性催化不对称杂环酮与芳香醛之间的Aldol反应。通过对溶剂、含水量、投料比、酶载量以及反应温度等条件的筛选，发现木瓜蛋白酶在该反应体系中展示了较好的催化活性和底物适应性。在最佳条件下，获得了最高37%的产率和78%的ee值。本文报道的木瓜蛋白酶在有机溶剂中具有类似天然醛缩酶的催化功能。 |
| 英文摘要: |
| The new promiscuous activity of papain, has been observed to catalyze the direct asymmetric Aldol reaction of heterocyclic ketones with aromatic aldehydes. We studied the effects of the solvent, the water content, the ratio of substrates, the enzyme loading and the temperature. Papain showed favorable catalytic activity and had a good adaptability to different substrates in the reaction. The yield of up to 37% and 78% ee were achieved under optimal conditions. It is interesting that papain possesses the function of aldolase in organic solvents. |
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