李晓璐,郭 晶,翟倩,易 钢.化学通报,2016,79(12):1127-1133.
核酸等温扩增技术在电化学生物传感器中的应用
The application of isothermal nucleic acid amplification technique in electrochemical biosensor
投稿时间:2016-05-16  修订日期:2016-07-01
DOI:
中文关键词:  核酸等温扩增技术,电化学分析,生物传感器,生物检测
英文关键词:isothermal nucleic acid amplification techniques  electroanalysis  biosensor  biodetection  
基金项目:
作者单位E-mail
李晓璐 重庆医科大学检验医学院 1260034290@qq.com 
郭 晶 重庆医科大学检验医学院  
翟倩 重庆医科大学检验医学院  
易 钢 重庆医科大学检验医学院 yigang666@126.com 
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中文摘要:
      生物分子检测在临床诊断、基因治疗、基因突变分析等方面变得日益重要,因而,建立简单、快速、灵敏的检测方法具有重要意义。近年,电化学生物传感器因其简单、便携、易操作、成本低等优势在生物分子检测的研究中备受关注。为了提高检测方法的灵敏度,不同的核酸等温扩增技术被应用于电化学生物传感器的构建中。本文简单介绍了电化学生物传感器的工作原理,着重综述了几种主要应用于电化学传感器中的核酸等温扩增技术,同时比较了各方法的优缺点。
英文摘要:
      The detection of biomolecules in clinical diagnosis, gene therapy and mutation analysis has become increasingly important. Therefore, it is significant for establishing a simple, rapid and sensitive detection method. Electrochemical biosensor has gained increasing interest because of its advantages such as simplicity, portable, easy to operate and low cost in the field of biomolecules detection. In order to improve the sensitivity of biosensor, the different isothermal nucleic acid amplification technique has been used in the construction of electrochemical biosensor. In this paper, we introducted the principle of electrochemical sensor briefly, summarize the main isothermal nucleic acid amplification techniques applied in electrochemical biosensors emphatically and compare the advantages and disadvantages of each technique.
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