| 苗向阳,郁惠珍,王杨,顾准,王兆寅,戴志晖.化学通报,2017,80(1):47-52. |
| Cu-MOF催化过氧化氢应用于多巴胺的检测 |
| DetectionSofSdopamineSbasedSonScatalyticSactivitySofSCu-MOFStowardsSH2O2 |
| 投稿时间：2016-07-04 修订日期：2016-08-25 |
| DOI： |
| 中文关键词: Cu-BTC 比色分析 过氧化氢 多巴胺 多相催化剂 |
| 英文关键词:Cu-BTC, Colorimetric analysis, H2O2, Dopamine, Heterogeneous catalyst |
| 基金项目:国家自然科学基金项目（21175069） |
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| 中文摘要: |
| 本工作利用一种具有过氧化氢(H2O2)催化活性的Cu-MOF (Cu3(BTC)2(H2O)3, 简称HKUST-1), 构建了一种以邻苯二胺 (OPD) 为颜色指示分子的比色传感体系, 实现了对H2O2和多巴胺(DA)的快速灵敏检测。HKUST-1起到催化H2O2氧化OPD的作用, 反应体系能够呈现出显著的颜色变化. 在最优条件下, 415 nm处的吸收峰强度与H2O2浓度呈双线性关系, 线性范围分别为10 mM至50 mM和50 mM至100 mM, 相对标准偏差分别为0.9947和0.9995, 最低检出限为1.29 mM. 由于DA能抑制H2O2氧化OPD, 因此比色传感体系还可以用于快速检测DA, 线性范围分别为0.25 μM至5 μM和2.5 μM至25 μM, 相对标准偏差分别为0.9783和0.9705, 最低检出限为0.262 μM. 该项工作拓展了Cu-MOFs材料在生物分子催化和生物传感方面的应用。 |
| 英文摘要: |
| In this work, a colorimetric biosensing platform for H2O2 and dopamine (DA) was constructed based on the catalytic activity of one type of Cu-MOF (Cu3(BTC)2(H2O)3, HKUST-1). The color of o-phenylenediamine (OPD) can be changed significantly because of the oxidation of H2O2 catalyzed by HKUST-1. As a result, H2O2 and DA were detected rapidly and sensitively by our system. Under the optimum conditions, two linear relationships between the intensity of absorption peak at 415nm and the concentration of H2O2 (from 10 to 50 mM and from 50 to 100 mM, R = 0.9947 and 0.9995) were obtained with the detection limit of 1.29 mM. Besides, DA can resist the oxidation of OPD from H2O2, this platform was further used for rapid detection of DA with two linear ranges (from 0.25 to 5μM and from 2.5 to 25μM, R = 0.9783 and 0.9705) and the detection limit of 0.262 μM. Therefore, this work may broaden the applications of Cu-MOFs in biological molecular catalysis and biosensing fields. |
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