| 孟二娟,桑延晓,祝智慧,刘事林,胡方中,任雪玲.化学通报,2017,80(10):953-957. |
| (E)-2-乙氧基乙基-2-氰基-3-((4-(2,4-二氟苯氧基)苄基)氨基)-3-甲氧基丙烯酸酯对超螺旋质粒DNA的特异性切割(英文） |
| Specific Cleavage of Supercoiled Plasmid DNA by (E)-2-Ethoxyethyl 2-Cyano-3-((4-(2, 4-Difluorophenoxy) benzyl)amino)-3-Methoxyacrylate |
| 投稿时间：2017-03-29 修订日期：2017-04-26 |
| DOI： |
| 中文关键词: DNA切割 丙烯酸乙酯 光谱 DNA测序 |
| 英文关键词:DNA cleavage, Acrylic ester, Spectroscopy, DNA sequencing |
| 基金项目:国家自然科学基金项目 |
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| 中文摘要: |
| 研究了一种新型丙烯酸酯 (E)-2-乙氧基乙基-2-氰基-3-((4-(2,4-二氟苯氧基)苄基)氨基)-3-甲氧基丙烯酸酯(MAZ)对超螺旋pUC19 DNA的定点切割作用。利用紫外吸收、荧光光谱、凝胶电泳和DNA测序技术研究其核酸切割特异性。紫外吸收光谱的红移和减色效应,以及静态荧光淬灭表明MAZ与DNA的作用属于典型的插入模式。不仅如此,凝胶电泳条带的变化以及DNA测序结果表明DNA的定点切割是通过DNA双链的分步水解完成。 |
| 英文摘要: |
| Here a novel cyanoacrylic ester, (E)-2-ethoxyethyl-2-cyano-3-((4-(2,4-difluorophenoxy) benzyl)amino)-3-methoxyacrylate (MAE), which is capable of site-specific cleavage of supercoiled pUC19 DNA, was introduced. The cleavage specificity was investigated through UV absorption, fluorescence, agarose gel electrophoresis, and DNA sequencing techniques. Red shift and hypochromism in the absorption spectra and static fluorescence quenching suggested that a typical intercalation occurred between DNA and MAE. In addition, detectable varieties in the gel electrophoresis and DNA sequencing indicated that the cleavage was site-specific and double strands of DNA were hydrolyzed step by step. |
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