| 周 希,刘美红,王春鹏,储富祥.化学通报,2018,81(1):65-69. |
| 水热法一步合成氮掺杂碳点及其应用于Cu(Ⅱ)离子检测 |
| One-step hydrothermal synthesis of nitrogen doped carbon dots and its application in Cu(Ⅱ) ion detection |
| 投稿时间：2017-07-29 修订日期：2017-09-05 |
| DOI： |
| 中文关键词: 碳材料 荧光 猝灭 细胞毒性 |
| 英文关键词:Carbon materials, Fluorescence, Quenching, Cell toxicity |
| 基金项目:中央级公益性科研院所基本科研业务费专项资助（Nos.CAFYBB2017ZX003）和中国林科院林业新技术所基本科研业务费专项资助（Nos.CAFYBB2017SY033）。 |
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| 中文摘要: |
| 采用水热法以聚乙烯亚胺为原料一步制备氮掺杂荧光碳点。紫外-可见吸收光谱、荧光光谱以及透射电镜显示,所制备的碳点荧光性能优异、分散性好、且无团聚现象。Cu(Ⅱ)对所得的碳点表现出选择性荧光猝灭效果,这种现象可用于Cu(Ⅱ)检测。在0.1 mol/L PBS溶液中,荧光碳点的荧光强度随着Cu(Ⅱ)浓度的增加逐渐减弱。该方法对Cu(Ⅱ)检测的线性范围为50~150 μmol/L,检出限为10 μmol/L。细胞毒性测试结果表明,不同浓度的碳点对细胞活性影响均较小,其细胞毒性低。以上结果说明该碳点能成功检测Cu(Ⅱ)且细胞毒性低,在生物传感方面有潜在应用价值。 |
| 英文摘要: |
| The nitrogen doped carbon dots based on polyethyleneimine were prepared by one-step hydrothermal method. Ultraviolet-visible absorption and fluorescence spectra and transmission electron microscopy show that the carbon dots have excellent fluorescence performace and good dispersion. Cu(Ⅱ) shows selective fluorescence quenching effect towards carbon dots, which can be used for Cu(Ⅱ) detection. In 0.1 mol/L PBS solution, the fluorescence intensity of carbon dots reduced as Cu(Ⅱ) concentration increases gradually. The detection linear range of Cu(Ⅱ) is 50-150 μmol/L with the detection limit of 10 μmol/L. Cytotoxicity test results show that different concentrations of the carbon dots all have low toxicity. The above results show that the carbon dots can successfully detect Cu(Ⅱ) and have low cytotoxicity, showing potential in biological sensing. |
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