| 刘严华,黄登发,朱锦雯,崔正刚,王峰.化学通报,2018,81(4):361-365,383. |
| L-苯丙氨酸聚肽的酶催化合成 |
| Enzymatic Synthesis of L-phenylalanine Polypeptide |
| 投稿时间：2017-09-23 修订日期：2018-01-18 |
| DOI： |
| 中文关键词: L-苯丙氨酸甲酯盐酸盐 酶催化 聚合 表征 |
| 英文关键词:L-phenylalanine methyl ester hydrochloride, Enzymatic Catalysis, Polymerization, Characterization |
| 基金项目: |
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| 中文摘要: |
| 聚肽是由人工合成的氨基酸聚合物，具有良好的生物相容性和生物可降解性。本研究以菠萝蛋白酶为催化剂，在温和条件下于非水体系中从L-苯丙氨酸甲酯聚合得到寡聚L-苯丙氨酸。通过FT-IR、1H-NMR、MS和MALDI-TOF确定了寡聚L-苯丙氨酸的结构和聚合度。表征结果证明合成产物为寡聚L-苯丙氨酸，最大聚合度为8。通过单因素考察了菠萝蛋白酶催化聚合反应的最优条件。结果表明：在40 ℃，用2.44 U菠萝蛋白酶于15% DMSO的磷酸缓冲液（pH=8.0）中催化0.43 g L-苯丙氨酸甲酯盐酸盐聚合，反应3小时后寡聚L-苯丙氨酸得率达到最大值，为35.67%。 |
| 英文摘要: |
| Artificial polymers of amino acids, polypeptides, show the advantages of biocompatibility and biodegradability. In this study, the oligo-L-phenylalanine was prepared by the polymerization reaction of bromelain from L- phenylalanine methyl ester in the non-aqueous media under the mild conditions. The structure and polymerization degree of the oligo-L-phenylalanine were elucidated by FT-IR, 1H-NMR, MS and MALDI-TOF. The characterizations indicated the product is oligo-L-phenylalanine with a maximal polymerization degree of eight. Finally, the optimum polymerization conditions were evaluated through single-factor approach. The highest value of yield, 35.67%, could be achieved when 0.43 g L-phenylalanine methyl ester hydrochloride in 15% DMSO phosphate buffer (pH=8.0) was polymerized by 2.44 U bromelain at 40 °C for 3 hours. |
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