| 陈成龙,孙澍雨,周维增,夏亚穆.化学通报,2018,81(10):896-902. |
| 漆酶在催化合成生物活性化合物中的研究进展 |
| Review on Applications of Laccase in Bioactive Compounds Synthesis |
| 投稿时间：2018-06-20 修订日期：2018-07-16 |
| DOI： |
| 中文关键词: 漆酶 生物催化 有机合成 活性化合物 |
| 英文关键词:Laccase, Biocatalysis, Organic synthesis, Bioactive compounds |
| 基金项目:国家自然科学基金项目（21472105） |
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| 摘要点击次数: 1862 |
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| 中文摘要: |
| 近年来市场对具有营养和药用价值的活性化合物的需求量逐年增加，尤其是在制药与食品行业。但是传统的生产方法已无法满足该类化合物的大规模应用。漆酶是近些年广受欢迎的生物催化剂之一，它可以在温和的条件下实现对活性化合物的高效合成，并且有极大潜力取代传统的工业生产方法。本文着重回顾了近十年来漆酶在催化合成活性化合物中的应用，并对漆酶的结构及作用机制进行了介绍。同时指出了漆酶工业化应用中存在的一些问题，比如漆酶产量不足、部分酶促反应介质不适于工业化应用等，并总结了通过异源表达、筛选高产菌株提高漆酶产量、使用固定化技术和蛋白质工程提高漆酶的使用寿命、开发更加高效低廉的反应介质系统与寻找新的漆酶底物相结合来降低漆酶的应用成本是今后主要的发展趋势。 |
| 英文摘要: |
| The increasing demand for bioactive compounds is mainly pushed by their wide range of nutritional and therapeutic benefits, especially in pharmaceutical and food industries. But the large-scale industrial application of bioactive compounds was limited by conventional production means. Laccase, as biocatalysts, is one of the encouraging potential enzymes for industrialization, which have demonstrated high efficiency in the synthesis of bioactive compounds under mild conditions. This review summarizes the application of this interesting enzyme in synthesizing bioactive compounds in the last decade, and also introduces the enzyme structure and the catalytic mechanism relevant to their application as biocatalysts. In addition, the review also comprises a discussion of the hurdles, such as lack of sufficient enzyme stocks and some laccase mediator systems are inapplicable for industrialization, etc, in industrialized application of laccase. Finally, the key areas for future research are pointed out which include enhancing output of laccase by heterologous expression, improving lifetime of laccase by immobilization and protein engineering and cutting cost of application by developing more effective and inexpensive mediator coupled with the search of new substrates. |
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