| 徐乐颜,王凝月,杨明玲,王小永.化学通报,2019,82(12):1104-1109. |
| 卡拉胶电荷量对牛血清蛋白/卡拉胶复合物包载姜黄素的增强作用研究 |
| Influence of Carrageenan Charge on the Encapsulation Properties of Curcumin in BSA/Carrageenan Complexes |
| 投稿时间：2019-06-25 修订日期：2019-08-03 |
| DOI： |
| 中文关键词: 姜黄素 蛋白质 多糖 静电吸引作用 疏水作用 |
| 英文关键词:Curcumin, Protein, Polysaccharide, Electrostatic attraction, Hydrophobic interaction |
| 基金项目:国家自然科学基金项目（21573071） |
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| 中文摘要: |
| 本文研究了带有不同负电荷量的κ-卡拉胶和λ-卡拉胶对牛血清蛋白/卡拉胶复合物包载姜黄素的增强作用。卡拉胶通过与牛血清蛋白之间的静电吸引作用，可以生成高稳定性的牛血清蛋白/卡拉胶复合物。相比纯牛血清蛋白，牛血清蛋白/卡拉胶复合物能够显著提高姜黄素的溶解度和稳定性。姜黄素的紫外和荧光光谱表明姜黄素主要是通过疏水作用缔合于牛血清蛋白的疏水微区。卡拉胶与牛血清蛋白的静电吸引作用能够稳定牛血清蛋白的折叠结构，从而使牛血清蛋白为姜黄素提供更加疏水的缔合微环境。相比纯牛血清蛋白和牛血清蛋白/κ-卡拉胶复合物，带有更高负电荷的λ-卡拉胶与牛血清蛋白生成的牛血清蛋白/λ-卡拉胶复合物与姜黄素有更大的缔合常数和pKa1。 |
| 英文摘要: |
| This work studies the influence of the different negative charges of κ-carrageenan and λ-carrageenan on the encapsulation properties of curcumin in BSA/carrageenan complexes. BSA/carrageenan complexes with high stability are formed through the electrostatic attraction between BSA and carrageenan. Compared to pure BSA, BSA/carrageenan complexes can significantly increase the solubility and stability of curcumin. Absorption and fluorescence measurements indicate that curcumin is bound in the hydrophobic cavities of BSA through the hydrophobic force. The presence of carrageenan can stabilize the folded structure of BSA for providing curcumin with a more hydrophobic microenvironment. Compared to pure BSA and BSA/κ-carrageenan complexes, the values of binding constant and pKa1 are bigger for curcumin with BSA/λ-carrageenan complexes, where λ-carrageenan carries higher negative charge than κ-carrageenan. |
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