| 马志莹,高雪,宋宇,刘秀英,励建荣.化学通报,2020,83(7):659-664. |
| 酪胺修饰石墨烯量子点检测血清中游离胆固醇 |
| Determination of Free Cholesterol in Serum by Tyramine Modified Graphene Quantum Dots |
| 投稿时间：2019-11-29 修订日期：2020-01-26 |
| DOI： |
| 中文关键词: TYR-GQDs H2O2 胆固醇 |
| 英文关键词:TYR-GQDs hydrogen peroxide cholesterol |
| 基金项目:国家自然科学基金项目（31501564）资助 |
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| 中文摘要: |
| 以柠檬酸为原料合成具有模拟过氧化氢酶活性的石墨烯量子点（GQDs），经酪胺修饰的GQDs（TYR-GQDs）可以将过氧化氢还原成羟基自由基，通过酚羟基之间的交联聚集，GQDs的荧光被猝灭。由于胆固醇氧化酶可以催化胆固醇氧化生成H2O2，因此，基于TYR-GQDs建立了一种检测胆固醇的荧光传感器。在pH=7.4的条件下，TYR-GQDs的荧光猝灭率与胆固醇浓度（2.67×10-8~2.67×10-3mol/L）的对数呈良好的线性关系，检出限为9.32×10-9mol/L。干扰实验表明，该荧光传感器对胆固醇具有高度的选择性，可以用于人体血清中游离胆固醇的检测，加标回收率为96.55%~100.14%。 |
| 英文摘要: |
| In this study, graphene quantum dots (GQDs) with catalase activity were synthesized from citric acid. GQDs modified by tyramine (TYR-GQDs) can reduce hydrogen peroxide to hydroxyl radicals. GQDs aggregates through the cross-linking between phenolic hydroxyls, which leads to fluorescence quenching. Since cholesterol oxidase can catalyze the oxidation of cholesterol to produce H2O2, a fluorescence sensor for detecting cholesterol was established based on TYR-GQDs. The experimental results showed that under the condition of pH=7.4, the fluorescence quenching rate of TYR-GQDs exlibits a good linear relationship with the logarithm of cholesterol concentration (2.67×10-8~2.67×10-3 mol/L), and the detection limit is 9.32×10-9 mol/L. The interference experiment showed that the fluorescent sensor has high selectivity for cholesterol and can be used for the detection of free cholesterol in human serum. The recovery of standard addition is 96.55%~100.14%. |
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