| 黎瑜辉,尹芳菲,李江,李兰英,刘刚.化学通报,2020,83(9):770-776. |
| 框架核酸在蛋白精确组装中的应用 |
| Application of Framework Nucleic Acids in Protein Precise Arrangement |
| 投稿时间：2020-01-15 修订日期：2020-04-22 |
| DOI： |
| 中文关键词: 框架核酸 DNA纳米技术 DNA折纸 蛋白精确组装 |
| 英文关键词:Framework Nucleic Acids DNA nanotechnology DNA origami Protein precise arrangement |
| 基金项目:“国家质量基础的共性技术研究与应用”重点专项（2017YFF0204603）、国家重点研发计划项目（2016YFA0201200）、国家自然科学基金项目（21834007，21675167，31571014，21775157，11575278）、上海市科学技术委员会项目（19JC1410302）、中国科学院重大科技基础设施开放研究项目、中国科学院王宽诚率先人才计划-卢嘉锡国际团队项目和上海交通大学资助 |
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| 中文摘要: |
| 除了作为遗传信息的载体，DNA所展现出的特殊材料性能引起了广泛关注。基于碱基互补配对原则的精确性和可编程性使得核酸纳米结构的构建逐步从一维单链发展到二维平面以及三维立体结构。计算机辅助工具的进步也促进了各种大小和形状的DNA纳米结构的自动化设计，而近年来构建的“框架核酸（FNAs）”为生物大分子纳米尺度上的精确排列提供了新方法，其固有的生物学功能以及可定制的特性使得其在物理、化学和生物等领域具有十分广阔的应用前景。本文阐述了精确自组装的FNAs的概念，并概述了FNAs在蛋白精确组装等领域的最新进展；重点论述了FNAs的优势所带来的对蛋白空间排布及其性能的调控能力，讨论了该领域存在的挑战，并对其发展机遇进行了展望。 |
| 英文摘要: |
| In addition to being the carrier of genetic information, DNA has attracted wide interest as a new class of material in the synthetic realm. The exploitation of the precise and programmable Watson-Crick base pairing of DNA or RNA has led to the development of exquisite nucleic acid nanostructures from one to three dimensions. Advances in computer-aided tools have also facilitated the automated design of DNA nanostructures with various sizes and shapes. The “framework nucleic acids (FNAs)” constructed in recent years provide a new method for precise organization of biological macromolecules with nanometer precision. The intrinsic biological properties and tailorable functionalities of FNAs hold great promise for physical, chemical, and biological applications. This review elaborates the concept of precise self-assembled FNAs, and summarizes the recent advances of FNAs in the field of protein precise arrangement. The unique features of FNAs that benefit the arrangement of proteins and their performance are highlighted. The challenges and opportunities of this field are also discussed. |
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