| 田佳琦,陈月荣,刘铭,张艳华,孙旭东,付尧,张秀凤,石磊.化学通报,2020,83(11):1025-1030. |
| 基于菁染料响应G-四链体结构变化的半胱氨酸检测探针 |
| A Cysteine Detection Probe Based on Regulating Cyanine Dye Transformation by G-quardruplex |
| 投稿时间：2020-04-11 修订日期：2020-07-17 |
| DOI： |
| 中文关键词: DNA G-四链体 菁染料 巯基蛋白 半胱氨酸 紫外光谱 |
| 英文关键词:DNA G-quadruplex Cyanine dyes Thiol protein Cysteine UV spectrum |
| 基金项目:华北理工大学大学生创新创业训练计划项目（X2018166）和国家自然科学基金项目（21807034）资助 |
|
| 摘要点击次数: 1186 |
| 全文下载次数: 0 |
| 中文摘要: |
| 半胱氨酸是生物体中起着重要作用的还原性氨基酸，其在体内的含量变化可能会诱发机体发生多种病变，因此高选择性、高灵敏度和低成本的半胱氨酸检测技术具有重要意义。本文利用富含鸟嘌呤的DNA链序列在钾、钠等金属离子诱导下形成对汞离子产生特殊响应的二级结构以及菁染料能够对DNA结构进行识别，并由此引起其超分子聚集形式的改变，致使其紫外和可见光谱性质随之变化，通过以Hg2+调控G-四链体与菁染料（ETC）组建传感器，实现对溶液体系中半胱氨酸高选择性检测。 |
| 英文摘要: |
| Cysteine is a reducing amino acid that plays an important role in organisms, and changes of its content in the body may induce a variety of lesions. Therefore, cysteine detection technology with high selectivity, high sensitivity and low cost is of great significance. In this paper, a guanine-rich DNA sequence is induced by potassium and sodium metal ions to form a secondary structure that has a special response to mercury ion. The cyanine dye can recognize the DNA structure, and thus cause the transformation of the supramolecular aggregation, resulting in changes in its UV and visible spectral properties. Finally, the sensor composed of Hg2+ regulating G-quadruplex and cyanine dye is utilized in the highly selective detection of cysteine in the solution system. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |