| 高永光,贾静娴,王丽红,张红霞,陈伟.化学通报,2021,84(8):829-834,819. |
| 罗丹明B修饰的树形高分子作为核酸递送载体的研究 |
| Rhodamine B modified dendrimers as efficient nucleic acid delivery vectors |
| 投稿时间：2020-12-30 修订日期：2021-01-31 |
| DOI： |
| 中文关键词: 罗丹明B 树形高分子 基因转染 基因载体 |
| 英文关键词:Rhodamine B Dendrimer Gene transfection Gene vectors |
| 基金项目:唐山师范学院科学研究基金项目(2021A03)和陕西省自然科学基金项目(2019JM-230)资助 |
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| 中文摘要: |
| 设计合成了三种罗丹明B修饰的树形高分子核酸递送载体GR-1、GR-2和GR-3,并通过核磁共振氢谱分析了树形分子表面联接的罗丹明B的数目。琼脂糖凝胶电泳实验表明,树形分子表面的罗丹明B数目越少,对RNA的浓缩效果越好。在三种核酸递送材料中,GR-3对Antagomir-138-5p的递送效率最高,基因沉默效率70% 左右,高于商业化的转染试剂Lipofectamine 2000。 |
| 英文摘要: |
| Three dendrimers (GR-1, GR-2 and GR-3) modified with rhodamine B were designed and synthesized. The number of rhodamine B conjugated to the periphery of dendrimers was determined using nuclear magnetic resonance spectroscopy (1H NMR). The agarose gel electrophoresis showed that the miRNA retardation ability was obviously improved with the decreasing number of rhodamine B on the surface of PAMAM dendrimers. Among the three materials, GR-3 showed the best transfection efficacy in RNA-mediated gene delivery, and gene silence efficacy was up to 70%, which was higher than the commercial transfection agent Lipofectamine 2000. |
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