| 徐夏帆,王帆,文晓秋,贺依,谭金梅,马林.化学通报,2021,84(10):1074-1079,1101. |
| 亚精胺对溶菌酶构象及催化性能的影响 |
| Conformational changes and bioactivity inhibition of lysozyme on the interaction with spermidine |
| 投稿时间：2021-01-29 修订日期：2021-04-02 |
| DOI： |
| 中文关键词: 溶菌酶 亚精胺 相互作用 构象 催化活性 |
| 英文关键词:Lysozyme, Spermidine, Interaction, Conformation, Catalytic activity |
| 基金项目:国家自然科学基金项目(21373062)资助 |
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| 中文摘要: |
| 聚乙烯亚胺是典型的聚阳离子基因载体材料，但是具有一定的细胞毒性，以小分子多胺为基础制备易降解的高分子材料是降低聚乙烯亚胺类基因载体材料的细胞毒性和提高基因转染效率的重要方法。为深入了解聚乙烯亚胺类基因载体材料与生物大分子的相互作用，论文综合应用吸收光谱、共振瑞利散射、圆二色谱和荧光光谱研究典型小分子多胺化合物亚精胺与溶菌酶的相互作用及其对酶的构象、催化活性和催化动力学的影响和机理。亚精胺是两亲性分子，其氨基基团易于质子化而具有较强的静电结合和氢键形成能力，而亚甲基基团具有一定的疏水性。亚精胺较小的分子尺寸和两亲性使其可以穿透进入溶菌酶的疏水核心，与溶菌酶肽链的非特异性结合导致溶菌酶二级结构由?-螺旋向?-折叠的转变，并导致溶菌酶表面疏水性轻微增强。亚精胺与溶菌酶的结合降低了溶菌酶对底物溶壁微球菌的亲和力和最大反应速率，低浓度的亚精胺对溶菌酶的活性具有显著的抑制作用。 |
| 英文摘要: |
| To get a further insight into the interaction between gene transfer vector based on polyamines and macromolecules, absorption, resonance Rayleigh scattering, fluorescence and circular dichroism were conducted to investigate the interaction between spermidine (Spd) and lysozyme (Lys) and the effects on the conformation and catalytic activity of the enzyme. Spd is a typical polyamine of hydrogen bonding capability and hydrophobicity due to amine groups and methyl groups. Due to the small size and the amphiphilic nature, Spd was found to penetrate into the hydrophobic core of Lys and bind to the aromatic residues and polypeptide chain without specific site, resulting in a conformation transition from ?-helix to ?-sheet and an enhancement of surface hydrophobicity of Lys. The interaction with Spd and the conformational change of Lys led to a decrease in the affinity towards Micrococcuslysodeikticus and the maximum rate of lysis reaction, exhibiting strong inhibition to enzymatic activity at low Spd concentration. |
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