| 刘成金,陶海燕,刘海燕.化学通报,2021,84(12):1383-1386. |
| CdTe水溶性量子点的合成表征及生物毒性测定——一个化学生物学实验 |
| Synthesis and Characterization of CdTe Water-soluble Quantum Dotsand Its Bio-toxicity Determination |
| 投稿时间：2021-02-25 修订日期：2021-05-22 |
| DOI： |
| 中文关键词: 量子点 合成 荧光量子产率 生物毒性 化学生物学实验 |
| 英文关键词:Quantum dots, Synthesis, Fluorescence quantum yields, Biological toxicity, Biochemical experiments |
| 基金项目:武汉大学实验技术项目(WHU-2020-XYKF-12)资助 |
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| 中文摘要: |
| 本实验以反应时间为变量,一步水相法合成了具有不同粒径的CdTe量子点。通过紫外-可见吸收光谱和荧光光谱表征不同反应时间得到的量子点,并计算其粒径、浓度以及荧光量子产率。取表征最优的量子点组次,配制成不同浓度,与酵母混合培养,用Biomass法测定CdTe量子点生物毒性。该实验涵盖无机合成相关知识,涉及分析表征方法应用以及量子点对生物细胞活性的影响,化学与生物学科交叉融合,有助于学生科研能力的培养与实验综合能力的提升。 |
| 英文摘要: |
| In this experiment, CdTe quantum dots were synthesized by one-step method in aqueous solution at different reaction time. The ultraviolet-visible spectrum and fluorescence spectra were used to characterize the obtained materials. According to the spectrum, the diameter, concentration and fluorescence quantum yields of CdTe quantum dots can be calculated. Then, the biological toxicity of CdTe quantum dots was determined by biomass method by mixing the different concentrations of CdTe quantum dots with yeast cells. This experiment covering inorganic synthesis, characterization and biochemistry, it’s helpful for improving students’ scientific research ability and experimental comprehensive ability. |
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