| 岳琳琳,彭鑫鑫,李巧凤,张国军,杨帆.化学通报,2022,85(2):169-176. |
| 核酸适配体功能化纳米界面用于循环肿瘤细胞捕获与释放 |
| CaptureandReleaseofCirculatingTumorCellsattheAptamerFunctionalizedNano-Interface |
| 投稿时间：2021-06-02 修订日期：2021-07-06 |
| DOI： |
| 中文关键词: 循环肿瘤细胞 纳米结构界面 核酸适配体 细胞捕获 细胞释放 |
| 英文关键词:circulating tumor cells nanostructured interface aptamers cell capture cell release |
| 基金项目:国家自然科学基金项目(21775034,21974037)资助 |
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| 中文摘要: |
| 循环肿瘤细胞(CTCs)是肿瘤研究和临床癌症诊断中的重要对象,也是“液体活检”的重要标志物。CTCs携带着肿瘤组织的遗传和表型信息,有助于肿瘤的早期诊断、个体化治疗和预后监测。然而,CTCs是一种极其罕见的细胞群体,在癌症患者外周血中十分稀少,这对从患者血液中分离CTCs并无损释放进行下游分析提出了挑战。目前,基于CTCs的物理和生物学特性已经发展了许多分离和富集CTCs的策略。为了克服目前CTCs捕获的常规界面局限性,具有独特性能的纳米界面应运而生,特别是利用多功能的“化学抗体”核酸适配体作为细胞识别元件。在此,综述了适配体功能化的多维(从零维到三维)纳米结构界面分离和释放CTCs的重要进展,并讨论了纳米界面与微流控相结合的优势。 |
| 英文摘要: |
| circulating tumor cells (CTCs) are not only the important object in tumor research and clinical cancer diagnosis, but also an important marker of "liquid biopsy". CTCs carry genetic and phenotypic information of tumor tissues, which are helpful for early diagnosis, prognosis monitoring and individualized treatment of tumor. However, CTC is an extremely rare cell population in the peripheral blood of cancer patients, which poses a challenge for their isolation and undamaged release from patients" blood and for downstream analysis. Currently, many strategies for isolating and enriching CTCs have been developed based on the physical and biological characteristics of CTCs. To overcome the limitations of the conventional macroscale interface, the nanostructured interface with unique properties has been deployed in CTCs capture and release, particularly by using multifunctional “chemical-antibodies”-aptamers as cell-recognition elements. In this review, we summarized the progress in the separation and release of CTCs based on nanostructured interface with nano-architectures from the zero dimensional (0D) to 3D, and discussed the advantages of the combination of nanointerface and microfluidics. |
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