| 杨丽霞,易姿,黄小贝,汪胜.化学通报,2022,85(8):971-975. |
| 基于发夹结构DNA铜纳米簇的链霉素快速检测 |
| Rapid Detection of Streptomycin Based on Hairpin DNA Copper Nanoclusters |
| 投稿时间：2021-12-17 修订日期：2022-01-06 |
| DOI： |
| 中文关键词: 铜纳米簇 无标记 发夹结构DNA 链霉素 |
| 英文关键词:copper nanoclusters,label free,hairpin DNA,streptomycin |
| 基金项目:湖南省自然科学基金科药联合基金项目(2021JJ80077)、国家市场监督管理总局科技计划项目(2020MK074)和湖南省市场监督管理局科技计划项目(2020KJJH39)资助 |
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| 中文摘要: |
| 建立了基于铜纳米簇（copper nanoclusters，CuNCs）的免标记荧光生物传感器快速检测链霉素的方法。利用链霉素核酸适配体和双链茎设计并合成了发夹结构DNA（hairpin DNA，hpDNA）模板，富含AT的双链茎部分可以合成CuNCs。在CuNCs荧光传感体系中加入链霉素后，链霉素与核酸适配体结合，使hpDNA双链茎打开，CuNCs荧光强度明显下降。在最优条件下，该方法检测链霉素的线性范围为0.1~20 mg/L，线性回归方程为y=240.17-10.31x，线性回归系数r2为0.9954，检出限为4.36 μg/L。该方法对牛奶样品的加标回收率为98.6%~104.8%。方法无需复杂的DNA序列设计、荧光标记，实验操作简单易行，适用于链霉素的快速检测。 |
| 英文摘要: |
| A rapid and label-free fluorescent sensing detection method has been developed for streptomycin by using copper nanoclusters biosensor. The hairpin DNA (hpDNA) template contained a streptomycin aptamer loop and a AT-rich double strand stem, and the AT-rich sequences are the template for the formation of fluorescent CuNCs. When streptomycin is present in the hpDNA-CuNCs solution, the fluorescence intensity decreased effectively, due to the structure switching of the hpDNA. Under optimized conditions, the method showed a good linear relationship with the streptomycin concentration in the range of 0.1-20 mg/L with correlation coefficients (r2) 0.9954. The detection limit of this method was 4.36 μg/L. Furthermore, the method was successfully applied to detect the streptomycin in milk samples and showed recoveries ranging 98.6%-104.8%. The method didn’t need complex DNA sequence design and was free of fluorescent labeling, and the operation is simple. Therefore, it is suitable for the rapid detection of streptomycin. |
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