冯珣,范荣华,邓丽丽,陈阳.化学通报,2022,85(11):1363-1370.
基于金属有机骨架衍生的CuO纳米酶比色法检测多巴胺
Colorimetric Detection of Dopamine Based on Metal-Organic Framework-Derived CuO Nanozymes
投稿时间:2022-04-04  修订日期:2022-05-05
DOI:
中文关键词:  氧化铜  纳米酶  比色分析  多巴胺
英文关键词:CuO  nanozyme  Colorimetric detection  Dopamine
基金项目:辽宁省自然科学基金项目(2020-MS-310)资助
作者单位E-mail
冯珣 沈阳医学院 公共卫生学院 辽宁 沈阳 fengxundfx@163.com 
范荣华 沈阳医学院 公共卫生学院 辽宁 沈阳  
邓丽丽 沈阳医学院 公共卫生学院 辽宁 沈阳  
陈阳 中国医科大学 药学院 辽宁 沈阳  
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中文摘要:
      本文通过简单热裂解由硝酸铜和1,3,5-苯三酸合成的铜基有机骨架材料制备了一种CuO 纳米材料。利用扫描电镜、透射电镜、X 射线衍射及X射线光电子能谱对CuO 的结构及元素组成进行了表征。该CuO纳米材料表现出良好的过氧化物酶活性,在H2O2 存在下,可以催化氧化无色底物3,3’,5,5’-四甲基联苯胺(TMB)变成蓝色产物。选择对苯二甲酸作为荧光探针验证了CuO的类酶催化机制,结果表明,CuO会催化H2 O2产生?OH,其可氧化TMB形成蓝色产物,而多巴胺的存在会抑制CuO对H2 O2氧化TMB的催化活性,从而实现对多巴胺的检测。该方法对多巴胺的检测具有良好的稳定性和催化活性,线性范围为5 ~ 50 μmol/ L和50~200 μmol/ L,检测限为0. 5μmol/ L。该比色传感器可以用于对人体尿液中的多巴胺的检测,表明该CuO 纳米酶在生物和临床诊断方面具有潜在的应用价值
英文摘要:
      In this paper, CuO nanomaterial were synthesized by simple pyrolysis of Cu-based organic framework material (Cu-MOF) synthesized from copper nitrate and 1, 3, 5-benzenetricarboxylic acid. The structure and elemental composition of CuO were characterized by SEM, TEM, XRD and XPS. The CuO nanomaterial exhibitsgood peroxidase-like activity and can catalyze the oxidation of colorless substrate 3,3′,5,5′-tetramethylbenzidine (TMB) to blue product in the presence of hydrogen peroxide (H2O2). The enzyme-like catalytic mechanism of CuO was studied by fluorometry using terephthalic acid as fluorescent probe. The result showed that CuO can catalyze H2O2to produce hydroxyl radical, and hydroxyl radical can oxidize TMB to form blue product. Oxidative TMBdiscoloration of CuO in the presence of H2O2can provide an effective platform for colorimetric detection of dopamine.The presence of dopamine can inhibit the catalytic activity of CuO for the oxidation of TMB by H2O2, thus realizing the detection of dopamine. This method had good stability and catalytic activity for the detection of dopamine. The linear range is 5~50 μmol/ L and 50~200 μmol/ L, and the detection limit is 0. 5 μmol/ L. This colorimetric sensor can be used for the detection of dopamine in human urine, indicating that CuO nanozyme has potential applicationvalue in biological and diagnostic applications.
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