| 周晓彤,许俊洋,姜艳军,马丽,周丽亚,郑晓冰,贺莹.化学通报,2023,86(9):1103-1111. |
| 铈基介孔MOF固定化酶的构建及催化性能研究 |
| Construction and catalytic performance of cerium-based mesoporous MOF immobilized enzymes |
| 投稿时间：2023-01-07 修订日期：2023-02-23 |
| DOI： |
| 中文关键词: 金属有机骨架(MOF) 铈基介孔材料 固定化酶 脂肪酶 |
| 英文关键词:Metal organic framework(MOF) Ce-based mesoporous materials enzyme immobilization lipase |
| 基金项目:国家自然科学基金项目(22178083和21878068)和河北省重点研发计划项目(21372804D和20372802D)资助 |
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| 中文摘要: |
| 金属有机骨架(Metal-organic framework,MOF)材料由于其孔隙率高、比表面积大以及具有发达的内联通孔道结构等优点,可以作为优良的生物分子固定化载体。通过表面活性自组装策略制备了铈基介孔MOF(Ce-MOF-F),表征结果表明,该材料有大的比表面积和呈辐射状的介孔孔道结构；以其为载体,南极假丝酵母脂肪酶B(CALB)为模型酶,通过物理吸附法,制备了生物催化剂CALB@Ce-MOF-F,对该固定化酶的酶载量和催化性能进行了研究。在优化条件下,CALB的负载量为162.03 mg/g载体,水解活性为899.1 U/g蛋白。与游离CALB相比,CALB@Ce-MOF-F表现出对高温、酸碱和有机溶剂等有更强的耐受性；将Ce-MOF-F用于多种酶的固定化,研究其作为载体的普适性,结果表明介孔Ce-MOF-F对洋葱伯克氏菌脂肪酶(BCL)和漆酶有良好的固定化效果,可以作为良好载体,并能对酶起到较好的保护作用。 |
| 英文摘要: |
| Metal-organic framework(MOF) materials have been used as excellent supports for immobilized enzymes due to their high porosity, large specific surface area and well-developed inline pore structure. The Ceria-based mesoporous MOF (Ce-MOF-F) had been prepared by surface-active self-assembly strategy. Its large specific surface area and pore structure were favorable for enzyme adsorption in Ce-MOF-F. The biocatalyst CALB@Ce-MOF-F was prepared by adsorption using Ce-MOF-F as the support and Candida antarctica lipase B (CALB) as the model enzyme. The enzyme loading and catalytic performance of the immobilized enzyme were investigated. Under the optimized conditions, the loading amount of CALB was 162.03 mg/gsupport, and the hydrolytic activity was 899.1 U/gprotein. CALB@Ce-MOF-F had exhibited greater tolerance to high temperature, acids, and alkalis as well as organic solutions compared to free CALB. Furthermore, Ce-MOF-F was used for the immobilization of various enzymes to study its general applicability as a carrier, and the results showed that Ce-MOF-F had good immobilization effect on immobilized Burkholderia cepacia lipase(BCL) and laccase, which proved that it could be a suitable carrier for the immobilization of enzymes and provide good protection. |
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