| 潘泓硕,徐嘉谊,陈信允,姜靓,徐香玉.化学通报,2024,87(3):361-366. |
| 基于纳米银的比色阵列传感器的构建及在蛋白质检测中的应用 |
| Construction of Colorimetric Array Sensor Basedon Silver Nanoparticles and Its Applicationin Protein Detection |
| 投稿时间：2023-06-14 修订日期：2023-08-22 |
| DOI： |
| 中文关键词: 纳米银 比色阵列传感器 蛋白质检测 |
| 英文关键词:Silver nanoparticles, Colorimetric sensor array, Protein detection |
| 基金项目:济宁医学院国家自然科学基金培育基金项目(JYP2018KJ03,JYP2018KJ17)、济宁医学院校级本科教学改革研究项目(yb202219)和济宁医学院大学生创新训练计划项目(cx2023053z,cx2023060z)资助 |
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| 中文摘要: |
| 蛋白质的快速高效检测和鉴定在医学诊断、不同疾病的治疗和蛋白质组学中具有巨大的前景。目前的检测手段大多存在一些问题，比如操作繁琐，效率低下等。因此开发一个理想的蛋白质检测方法尤为重要。以纳米银(silver nanoparticles, AgNPs) 为传感元件的阵列传感器在蛋白质检测方面具有操作便捷、准确率高、可视化等优点。本实验将合成两种不同颜色和形状的纳米银：黄色球形和蓝色三角形，以此构建一个简单的比色阵列传感器，用于蛋白质的区分检测。该传感器可以准确地识别和区分不同种类的蛋白质，准确率为100%。在成功识别出不同类型的蛋白质的基础上，进一步评估了该阵列传感器应用于区分正常和变性蛋白质的能力，准确率为96.0%。此外，该阵列传感器对于未知样本的识别也具有高的准确率。 |
| 英文摘要: |
| Efficient and rapid detection and characterization of proteins can aid in medical diagnosis, treatment of different diseases, and proteomics. However, the current assays often have issues such as low efficiency and complicated operation. Therefore, the need for an ideal protein detection method is vital. Array sensors that use silver nanoparticles (AgNPs) as the sensing element have become popular due to their easy operation, high accuracy, and visualization in protein detection. In this experiment, two AgNPs with different colors and shapes: yellow spheres and blue triangles, will be synthesized to construct a simple colorimetric array sensor for protein discrimination detection. This sensor has been successful in accurately recognizing and distinguishing various types of proteins with an accuracy rate of 100%. Additionally, the array sensor was evaluated for its ability to differentiate between normal and denatured proteins, achieving an accuracy rate of 96.0%. Furthermore, the array sensor can also accurately recognize and detect unknown samples. |
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