| 明紫轩,张国军,李玉桃.化学通报,2025,88(5):490-500. |
| 基于CRISPR/Cas系统的生物传感器研究进展 |
| Advance in CRISPR/Cas-based biosensors |
| 投稿时间：2024-10-07 修订日期：2024-12-31 |
| DOI： |
| 中文关键词: CRISPR/Cas 系统 生物传感器 电化学 荧光 |
| 英文关键词:CRISPR/Cas system, biosensor, electrochemistry, fluorescence |
| 基金项目:湖北省自然科学基金创新发展联合基金项目(2023AFD125)资助 |
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| 中文摘要: |
| 成簇规律间隔短回文重复序列及相关蛋白（CRISPR/Cas）系统作为下一代诊断工具，通常与聚合酶链式反应、重组酶聚合酶扩增、滚环扩增等多种技术结合，在基因编辑、病原体检测、即时检测等领域上应用广泛。基于CRISPR/Cas的生物传感器往往具有免扩增、灵敏度高、便携性、成本效益、操作简便等优点，是灵敏检测核酸、蛋白质、细菌等靶标的理想方法。本文对CRISPR/Cas系统的作用机制进行介绍，回顾了基于CRISPR/Cas系统的各类生??物传感器的最新研究进展及其应用，总结了基于该系统生物传感器检测的现状和挑战，希望可以帮助大家进一步了解其未来发展和应用潜力。 |
| 英文摘要: |
| CRISPR/Cas, as a cutting-edge diagnostic platform, is frequently integrated with technologies such as Polymerase Chain Reaction (PCR), Recombinase Polymerase Amplification (RPA), and Rolling Circle Amplification (RCA). These systems are extensively applied in the fields of gene editing, pathogen detection, and point-of-care testing. CRISPR/Cas-based biosensors are characterized by their amplification-free operation, high sensitivity, portability, cost-effectiveness, and ease of use, making them ideal for the sensitive detection of nucleic acids, proteins, bacteria, and other targets. Here, we introduce the workings mechanism of the CRISPR/Cas system, reviews the latest research progress and applications of various types of CRISPR/Cas-based biosensors, and summarizes the current status and challenges in their detection capabilities. We hope to provide readers with a deeper understanding of their future development and application potential. |
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