| 黄丽娜,王玉敏,李东晏,吴相华.化学通报,2022,85(7):839-844. |
| 一种1,8-萘酰亚胺衍生物检测半胱氨酸荧光探针 的合成及其在活细胞和秀丽隐杆线虫中成像中的运用 |
| Synthesis of a 1,8-naphthalimide derivative fluorescence probe for detecting cysteine and its application in imaging of living cells and Caenorhabditis elegans |
| 投稿时间：2021-09-28 修订日期：2022-01-10 |
| DOI： |
| 中文关键词: 1,8-萘酰亚胺 荧光探针 半胱氨酸 高选择性 荧光成像 |
| 英文关键词:1,8-Naphthalimide, Fluorescence Probe, Cysteine, High Selectivity, Fluorescence imaging |
| 基金项目:云南省科技厅科技计划项目(No. 202001AT070068 ) |
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| 中文摘要: |
| 本文基于1,8-萘酰亚胺衍生物，构建了一种检测半胱氨酸 (Cysteine, Cys)的新型荧光探针TPFC-Acryloyl。光谱研究表明该探针能有效识别Cys，且能够在1 min内实现快速响应。探针对Cys的检测表现出高选择性，检测极限为2.13 mol/L。经荧光光谱和质谱实验确证其检测机理为：Cys与TPFC-Acryloyl分子中的丙烯酸酯发生共轭加成-环化反应，进而羟基裸露的同时释放出黄色荧光。细胞毒性测试表明探针TPFC-Acryloyl的细胞毒性低。此外，该探针还被成功应用于活细胞和秀丽隐杆线虫中Cys的荧光成像。 |
| 英文摘要: |
| A novel fluorescent probe TPFC-Acryloyl based on 1,8-naphthalimide derivative was designed as the cysteine (Cys) detector. Spectral studies showed that the probe can effectively identify Cys and achieve rapid response within 1 min. The probe displayed high selectivity and strong anti-interference ability for the detection of Cys, and the detection limit was 2.13 mol/L. The response mechanism was that Cys reacted with the acrylic ester of TPFC-Acryloyl and the conjugated addition-cyclization reaction occurred, then the hydroxyl group was exposed and yellow fluorescence was released. The mechanism was confirmed by MS analysis. Cytotoxicity testing showed that TPFC-Acryloyl displayed low cytotoxicity. In addition, the probe was successfully applied to the fluorescence imaging of Cys in living cells and C. elegans. |
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